Around 30,000 people who travel on Wellington trains daily will be out of luck on Thursday.
Hundreds of workers are going on strike and Metlink says there will be no train services at any point during the day and no bus replacements.
The Rail and Maritime Union says workers employed by Transdev Wellington and Hyundai Rotem have voted overwhelmingly in favour of stopping work for 24 hours from 2am tomorrow morning.
It's the first industrial action since 1994 to affect the Wellington rail system for longer than two hours.
Rail and Maritime Union secretary Wayne Butson says 400 workers are walking off the job because their employer is trying to increase profits by squeezing frontline workers, including trying to remove long-standing conditions for collective agreements.
The New Zealand Transport Agency said commuters should plan ahead to avoid delays, as there will be heavy traffic on the roads while trains are out of operation.