The World Health Organisation says Liberia is free of the Ebola virus 42 days after the last confirmed case passed a second negative test.
"WHO declares Liberia free of Ebola virus transmission in the human population," the UN health agency said in a statement on Thursday (local time).
The WHO, which had previously declared Liberia Ebola-free in May only to see the deadly virus resurface six weeks later, said the country had now entered a 90-day period of heightened surveillance.
It hailed Liberia's "successful response" to the recent re-emergence of Ebola, when six people were infected, including two who died.
"Liberia's ability to effectively respond to the outbreak of Ebola virus disease is due to intensified vigilance and rapid response by the government and multiple partners," the WHO said.
Liberia was the hardest hit in the west African Ebola outbreak that began in December 2013 and infected more than 28,000 people. It claimed more than 11,000 lives, mainly in Liberia, Guinea and Sierra Leone.
More than 10,500 of those infections and 4800 of the deaths occurred in Liberia.
A country is considered free of Ebola transmission once two 21-day incubation periods have passed since the last known case tests negative for a second time.
But experts warn that even after 42 days have passed the danger is not over since some Ebola cases are still surfacing in neighbouring Guinea and Sierra Leone.
The Ebola virus has been found lingering in the semen of male survivors many months after they tested negative.