A representative for the 90s heartthrob confirmed that Perry, 52, had died at St Joseph's Hospital in California, surrounded by his family and close friends.
The stroke struck on the same day a Beverly Hills 90210 reboot was announced - the show which shot him to fame.
"My heart is broken," Breakfast Club star Molly Ringwald tweeted.
"I will miss you so much Luke Perry. Sending all my love to your family."
Actress Jennifer Love Hewitt simply posted an old photo of the actor on Instagram, accompanied by a series of broken heart emojis.
Charlie Sheen penned a poem for his old friend, which he shared on Twitter.
"L.P, you made every situation better, my man. Your elegance, your wit, your charm and your giant heart, inspired and enchanted so many of us, on countless occasions, with brilliant aplomb," he wrote.
The Riverdale writer's room also shared a moving message for Perry, who played Fred Andrews on the hit show.
"Luke Perry... you were a joyful and vibrant soul. You will be missed but most certainly your legacy will be remembered forever. Rest in love and peace, friend."
Perry's Riverdale co-star Lili Reinhart said she could "barely find the words" in a post to her Instagram story.
"I am devastated. We all are."
Kiwi actor KJ Apa, who plays Archie, the son of Perry's character on Riverdale, has yet to publicly address the news.