A lab test has failed to identify any tuna DNA in more than 125cm of Subway sandwiches.
The test, commissioned by The New York Times, tested all the "tuna" meat from a numerous number of sandwiches bought from three stores across Los Angeles.
Researchers were unable to pinpoint a species from which the meat supposedly originated.
"There's two conclusions," a lab spokesperson told the Times. "One, it’s so heavily processed that whatever we could pull out, we couldn't make an identification.
"Or we got some and there's just nothing there that's tuna."
It's not the first time people have suspected there's something fishy about the sandwiches. In February, a similar test was run with samples from New York.
This time, they did find some tuna. In January, a class action lawsuit against Subway claimed the sandwiches were "a mixture of various concoctions", ingredients "blended together … to imitate the appearance of tuna".
The complainants later walked the lawsuit back, but maintained the "labelling, marketing and and advertising" for the sandwiches was "false and misleading".
But the sandwich chain has staunchly defended its tuna, saying the recent lawsuit is "baseless".
Earlier in June the sandwich chain had a promotion offering a 15 percent discount for its tuna subs with the promotional code 'ITSREAL'.