The Ministry for Primary Industries (MPI) on Thursday morning confirmed the cattle disease had been detected at a dry stock beef farm.
The farm has been placed into lockdown, with no animals allowed in or out.
MPI says it tracked the disease there by tracing animals' movements from farms known to be infected.
MPI won't reveal which farm it is, but has informed the neighbours which are at "very low" risk of also getting infected.
M bovis, a bacteria, can cause a range of serious health conditions for affected cattle including untreatable mastitis, pneumonia, ear infections and swollen joints. It can be hard to contain because animals can carry the disease without showing any symptoms.
M bovis poses no risk to humans, but has the potential to cause millions of damage to New Zealand's important agricultural industry.