The Wallabies will be without Marika Koroibete, Pone Fa'amausili and Isi Naisarani for Saturday's Bledisloe Cup opener against the All Blacks at Eden Park, with the trio stood down for breaching team protocols.
Rugby Australia on Wednesday confirmed that the three players won't be considered for Dave Rennie's side to face the All Blacks, after breaching team protocols after a team dinner.
Koroibete was only cleared to face the All Blacks late last month, after his controversial red card from the third and final test against France was rescinded.
The three had been drinking the night before a team day off. Although no damage was caused and no complaints were made, coach Rennie has decided to rule the three players out of selection for Saturday.
"We are building a culture of working hard for each other and being accountable for our actions," he says.
"On this occasion, these three players haven't lived up to that and we've taken a very clear stance.
"Ultimately these three men have paid a big price for their decisions but that's the standard we expect of each other."
The Wallabies will name their side to face the All Blacks on Thursday.