Air New Zealand's first flight from Auckland to Invercargill in months will land at 12:40pm on Monday, with the return departing Invercargill at 1:25pm.
The route was shut down earlier in the year due to the impact of the COVID-19 pandemic.
Air NZ's head of tourism and regional affairs Reuben Levermore said the return of the service to Invercargill has been much anticipated by the Southland community.
"When we initially launched the jet service last year, we couldn't have asked for a more enthusiastic response from the Southland community who know full well the value of a direct connection to our country's largest city and international gateway."
Flights will operate on Monday, Thursday, Friday and Sunday.
The direct service brings Auckland closer to destinations such as Stewart Island, Fiordland and the Catlins coast.
Air NZ also resumed its Wellington-Invercargill route on Sunday, with one daily return service operating on the route using the airline's 50-seat Q300 aircraft.
There will also be two additional return services on this route each week of the July school holidays.
Flights between Invercargill and Christchurch resumed under COVID-19 alert level 2.